Background: Diagnosis of prostatic needle biopsies can be difficult due to
benign mimics of
carcinoma (e.g. atrophy) and borderline changes often termed
as atypical
small acinar proliferation (ASAP).
To discriminate these lesions from small
focus carcinomas, immunohistochemistry (IHC) can
be used to label basal cells of non-malignant
glands (antibodies to p63), and carcinoma cells
can be marked by alpha-methylacyl-CoA
racemase (AMACR).
For a definitive diagnosis pathologists need to compare these
immunostains
with tissue
morphology using standard hematoxylin and eosin staining (H&E). This
web page is a demonstration of a new technique
enabling comparison of H&E and IHC stains made sequentially on
the same slide. more
Study design: 30 H&E stained prostatic needle biopsies from
23 patients were digitized. The slides were destained and
then immunostained with a cocktail of AMACR and p63 antibodies.
The slides were scanned once more and the pairs of virtual slides were processed for simultaneous viewing,
layered or side-by-side.
more
Publications:
A complete description of the methodology, and the results of
this study has been published in the February 2006 issue
of The
Journal of Urology
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Click on a thumbnail image to view the
virtual slide (in a new window) |
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Case 1 |
Case 2 |
Case 3 |
Case 4 |
Case 5 |
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Case 1-5 |
6-11
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12-16
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17-23 |
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